Overview
- Overview
- Specimen
- Clinical & Interpretive
- Performance
- Fees & Codes
- Setup & Updates
Useful For
Suggests clinical disorders or settings where the test may be helpful
Profile Information
A profile is a group of laboratory tests that are ordered and performed together under a single Mayo Test ID. Profile information lists the test performed, inclusive of the test fee, when a profile is ordered and includes reporting names and individual availability.
| FRAGO | Osmotic Fragility | No | Yes |
| SCTRL | Shipping Control Vial | No | Yes |
Method Name
A short description of the method used to perform the test
Osmotic Lysis
NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Yes
Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test
Osmotic Fragility, RBC
Aliases
Lists additional common names for a test, as an aid in searching
FRAGILITY, OSMOTIC [RBC]
RBC Fragility, Erythrocytes
Red Cell Fragility
Specimen Type
Describes the specimen type validated for testing
Control
Whole Blood EDTA
Shipping Instructions
Specimens must arrive within 72 hours of collection.
Necessary Information
Patient's age and sex are required.
Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing
Both a whole blood EDTA specimen and a control specimen are required as temperature extremes can increase the fragility of the specimen and cause false-positive results.
Patient:
Container/Tube: Lavender top [EDTA]
Specimen Volume: 4 mL
Collection Instructions:
1. Immediately refrigerate specimen after collection. Refrigerate at 0 to 4 degrees C. Do not freeze. Freezing causes sample lysis, and tests will not be performed on hemolyzed specimens.
2. Send specimen in original tube. Do not aliquot.
3. Rubber band patient specimen and control vial together. Control must accompany the patient sample at all times to ensure the reliability of testing results.
4. Be sure specimen and control are stored and transported together at refrigerated temperature, carefully following proper handling and shipping instructions.
Normal Shipping Control:
Specimen Type: Whole blood
Container/Tube: Lavender top [EDTA]
Specimen Volume: 4 mL
Collection Instructions:
1. Draw a control specimen from a normal [healthy], unrelated, nonsmoking person at the same time as the patient.
2. Handwrite "normal control" clearly on the outermost label.
3. Immediately refrigerate specimen after collection. Refrigerate at 0 to 4 degrees C. Do not freeze. Freezing causes sample lysis, and tests will not be performed on hemolyzed specimens.
4. Send specimen in original tube. Do not aliquot.
5. Rubber band patient specimen and control vial together. Control must accompany the patient sample at all times to ensure the reliability of testing results.
Forms
Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory
2 mL
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
| Gross hemolysis | Reject |
| Clotted blood | Reject |
Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included
| Control | Refrigerated [preferred] | 72 hours | PURPLE OR PINK TOP/EDTA |
| Whole Blood EDTA | Refrigerated [preferred] | 72 hours |
Useful For
Suggests clinical disorders or settings where the test may be helpful
Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Spherocytes are osmotically fragile cells that rupture more easily in a hypotonic solution than do normal RBCs. Because they have a low surface area:volume ratio, they lyse at a higher osmolarity than do normal discocyte [RBCs]. Cells that have a larger surface area:volume ratio, such as target cells or hypochromic cells are more resistant to lysing. After incubation, an increase in hemolysis is seen in spherocytes. Hereditary spherocytosis typically has greater number of spherocytes than other causes of spherocytosis. Therefore, the degree of lysis is usually more pronounced, but this is not always the case. Some rare disorders can also cause marked fragility and hereditary spherocytosis cases can display moderate fragility.
Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
> or =12 months:
0.50 g/dL NaCl [unincubated]: 3-53% hemolysis
0.60 g/dL NaCl [incubated]: 14-74% hemolysis
0.65 g/dL NaCl [incubated]: 4-40% hemolysis
0.75 g/dL NaCl [incubated]: 1-11% hemolysis
Reference values have not been established for patients who are